Engineered to enable in vitro skin research in which fibroblast-keratinocyte cell interactions are important, hg8868皇冠国际的表皮ft系统由正常的, 人表皮角质形成细胞(NHEK)和正常, human dermal fibroblasts (NHFB) cultured to form a multilayered model of the human dermis and epidermis. Cultured at the air-liquid interface in easy-to-handle tissue culture inserts, EpiDermFT attains levels of differentiation at the cutting edge of tissue engineering technology.
表皮上皮由表达角蛋白5的有组织基底细胞组成, 表达棘层和颗粒层的总苞蛋白和角蛋白10, 以及类似于体内发现的角化表皮层. The dermal compartment is composed of a collagen matrix containing viable normal human dermal fibroblasts (NHDF). The epidermal and dermal layers are mitotically and metabolically active and exhibit in vivo-like morphological and growth characteristics which are uniform and highly reproducible.
A 基底膜发育良好 是否存在于真皮/表皮交界处. Hemidesomosomes(›), lamina lucida, lamina densa(→) and anchoring fibril structures(») are evident by transmission electron microscopy.
EpiDermFT tissues are used across a wide range of applications including scientific efficacy and claims substantiation. 由于皮肤表面干燥, EpiDermFT can be used to evaluate biological responses to topical applications of formulations or ingredients. Simple protocols and the evaluation of early molecular endpoints allow research to acquire data in days, not weeks or months.
Examine changes in extracellular matrix gene and protein expression and histological structure to determine the anti-aging efficacy of cosmetic ingredients or final formulation. 表皮上皮细胞可从一系列成人供体中获得.
使用表皮mft用伤口愈合测定试剂盒评估治疗方法. Wound healing assay kits include 24 pre-wounded tissues and are available with epidermal only wounds or full thickness wounds of the epidermis and dermis.
Use EpiDermFT to assess the efficacy of topically applied moisturizers by measuring the electrical impedance of the epidermis. Amenable to creams, gel, 乳液或其他配方, 皮肤水合试验允许在24小时内进行数据分析.
分析环嘧啶二聚体的形成, inflammatory mediator release and oxidative endpoints to evaluate the UV protective efficacy of compounds or formulations.
Browse our reference library 看看我们的表皮组织是如何在这些研究领域中使用的.
Kit: 一个标准的EpiDermFT试剂盒(EFT-400)由24个组织组成. (纸巾“包”包含纸巾, 少量培养基, and plasticware; contact hg8868皇冠国际 for specific kit contents)
Substrate: 使用Costar Snapwell™单孔组织培养板插入. Pore size = 0.4 μm, Diameter = 1.2 cm. Surface area = 1.0 cm2
Culture: Air-liquid interface
Histology: 8-12 cell layers plus stratum corneum (basal, spinous, and granular layers)
Lot numbers: 每周生产的纸巾批次都有一个特定的批号. A letter of the alphabet is appended to the end of the lot number to differentiate between individual kits within a given lot of tissues. 所有组织试剂盒在细胞方面都是相同的, medium, handling, culture conditions, etc.
Shipment day: Every Monday. 如有特殊要求，也可在星期四装运
Delivery: 周二上午通过联邦快递优先服务(美国). 美国以外:周二至周四视地点而定
Shelf life: Including time in transit, tissues may be stored at 4°C for up to 6 days prior to use. 但是，除非必要，否则不建议延长储存期限. In addition, the best reproducibility will be obtained if tissues are used consistently on the same day, e.g. Tuesday afternoon or following overnight storage at 4°C (Wednesday morning)
Length of experiments: Cultures can be continued for up to 2 weeks with good retention of normal epidermal morphology. 培养物必须每隔一天用5.0 ml of EFT-400 medium
Type: Normal human epidermal keratinocytes (NHEK); Normal human dermal fibroblasts (NHDF)
Genetic make-up: Single donor
Derived from: Neonatal-foreskin tissue (NHEK); Neonatal skin (NHDF)
Alternatives: NHEK from adult breast skin; NHDF from adult skin
Screened for: HIV，乙型肝炎，丙型肝炎，支原体
Base medium: Dulbecco 's Modified Eagle 's Medium (DMEM)
生长因子/激素: Epidermal growth factor, insulin, hydrocortisone and other proprietary stimulators of epidermal differentiation
Anti-fungal agent: Amphotericin B 0.25 µg/ml
pH Indicator: Phenol red
Other additives: Lipid precursors used to enhance epidermal barrier formation (proprietary)
Alternatives: 不含酚红(EFT-400-PRF), 不需抗生素(eft - 400沛富), anti-fungal-free (eft - 400等于off), 或无氢化可的松培养基和组织(EFT-400-HCF)可用. 代理商至少在发货前3天被移除
分析/维护媒介: EFT-400-ASY is utilized for assays; EFT-400-MM is used for term maintenance of the EFT-400 tissues
Visual inspection: All tissues are visually inspected and if physical imperfections are noted, 纸巾被拒绝装运
End-use testing: 将组织暴露于1% Triton X-100中8,12,16和24小时. The time of exposure required to reduce the tissue viability (ET-50) using the MTT viability assay is determined (See hg8868皇冠国际 EpiDermFT use protocol) for each lot of tissue. ET-50一般在6的范围内.5-9.5 hours. 客户实验室的ET-50可能与hg8868皇冠国际的结果略有不同
Sterility: 在整个生产过程中使用的所有介质都要进行无菌检查. Maintenance medium is incubated with and without antibiotics for 1 week and checked for sterility. The agarose gel from the 24-well plate used for shipping is also incubated for 1 week and checked for any sign of contamination
病原体筛查: All cells are screened and are negative for HIV, hepatitis B and hepatitis C using PCR. However, no known test method can offer complete assurance that the cells are pathogen free. Thus, these products and all human derived products should be handled at BSL-2 levels (biosafety level 2) or higher as recommended in the CDC-NIH manual, 微生物和生物医学实验室的生物安全,” 1998. For further assistance, please contact your site Safety Officer or hg8868皇冠国际 technical service
不合格批件通知: 如果纸巾没有通过我们的质量检验或无菌检验, the customer will be notified and the tissues will be replaced without charge when appropriate. 因为我们的QC和无菌检测是在出货后完成的, 在一般情况下，我们会尽快发出通知, ET-50故障将在周三下午5点前通知.m.; sterility failures will be notified within 8 days of shipment)
To ensure that the most appropriate products and accessories for your application are included with your initial order, hg8868皇冠国际 strongly recommends a complimentary consultation with one of our highly trained Technical Specialists. 请联系Jonathan Oldach订购表皮mft产品.
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