EpiAirway • hg8868皇冠国际 Life Sciences-hg8868皇冠国际平台

EpiAirway is a ready-to-use, 3D mucociliary tissue model consisting of normal, 人源性气管/支气管上皮细胞也可与正常人间质成纤维细胞(EpiAirwayFT)共培养。. Cultured at the air-liquid interface (ALI), EpiAirway recapitulates the in vivo phenotypes of barrier, mucociliary responses, infection, toxicity responses and disease. With over 100 technical references available, EpiAirway适用于急性或长期慢性研究，在广泛的体外应用中具有高度预测性.

EpiAirway 6-week Culture — EpiAirway at 6 weeks in culture.

EpiAirway具有与人类相关的组织结构和细胞形态，具有高度的均匀性和可重复性. Its 3D structure consists of organized Keratin 5+ basal cells, mucus producing goblet cells, functional tight junctions and beating cilia. EpiAirwayFT将人成纤维细胞纳入细胞外基质，是炎症和纤维化研究的理想选择.

EpiAirway 3D人体组织模型通常用于包括安全在内的一系列应用 & risk assessment, anti-viral research, inflammation & fibrosis and drug delivery. 简单的协议和早期细胞端点的评估使科学家能够在几天内获得数据, not weeks or months.

Virus Infection

Use EpiAirway to study respiratory virus attachment, replication, innate immune responses and anti-viral drug development.

Viral Infection Reference

Drug Delivery

利用现有的透皮渗透设备或hg8868皇冠国际的简单单插入渗透设备来评估API的渗透和通量.

Drug Delivery Protocol

Drug Delivery Reference

Inhalation Toxicology

使用EpiAirway(可用于生理暴露系统的几种格式)通过EpiAirway IC-75测定来确定相对安全性.

EpiAirway Toxicity Protocol

Inhalation Toxicology Reference

Inflammation and Fibrosis

使用EpiAirway的健康和患病供体的多样化供体清单(哮喘, COPD, Smoker, 杯状细胞增生)来阐明分子途径并评估治疗效果.

View the hg8868皇冠国际 Reference Library

Tissue

Kit: A standard EpiAirway kit (AIR-100) consists of 24 tissues. (Tissue “kits” contain tissues, a small amount of culture medium, and plasticware; contact hg8868皇冠国际 for specific kit contents)

Formats:

9mm & 22mm的个体插入-组织培养基质经化学修饰，孔径为0.4 μm
24mm, 12mm & 6.5mm Transwell inserts with a pore size of 0.4 μm
96孔HTS板-组织培养底物经化学修饰，孔径为0.4 μm

Culture: Air-liquid interface

Histology: 3-4层细胞-假层状，毛粘形态和组织结构

Lot numbers: Tissue lots produced each week are assigned a specific lot number. 在批号的末尾附加一个字母，以区分给定批号中的单个试剂盒. All tissue kits within a lot are identical in regards to cells, medium, handling, culture conditions, etc.

Shipment: At 4°C on medium-supplemented, agarose gels

Shipment day: Every Monday

Delivery: Tuesday morning via FedEx priority service (US). Outside US: Tuesday-Thursday depending on location

Shelf life: 包括运输时间在内，纸巾在使用前可在4°C下保存3天. 但是，除非必要，否则不建议延长储存期限. In addition, 如果组织在同一天连续使用，将获得最佳的再现性, e.g. 周二下午或之后在4°C下过夜(周三上午)

Length of experiments: 组织培养可以持续三(3)个月或更长时间，保持良好的正常形态. Tissues must be fed every other day with 5.0 ml of maintenance medium (AIR-100-MM). 细胞培养插入放置在6孔板上的垫圈(EPI-WSHR)或培养架(MEL-STND)上，以允许我们5.0 ml. See technical reference #631.

Cells

Type: Normal human tracheal/bronchial epithelial cells (NHBE);

Genetic make-up: Single donor

Derived from: Healthy, non-smoker (alternate donors available upon request)

Alternatives: NHBE from Asthmatic, COPD and Smoker donors

Screened for: HIV, Hepatitis-B, Hepatitis-C, mycoplasma

Medium

Base medium: Dulbecco’s Modified Eagle’s Medium (DMEM)

Growth factors/hormones: Epidermal growth factors and other proprietary factors

Serum: None

Antibiotics: Gentamicin 5 µg/ml (10% of normal gentamicin level)

Anti-fungal agent: Amphotericin B 0.25 µg/ml

pH Indicator: Phenol red

Other additives: Proprietary

Alternatives: 无酚红，无抗生素，无真菌的培养基和组织. Agents are removed at least 3 days prior to shipment.

Assay medium: AIR-100-ASY

Maintenance medium: AIR-100-MM

Quality Control and Sterility

Visual inspection: 所有组织都经过目视检查，如果发现物理缺陷, tissues are rejected for shipment

End-use testing: 每个EpiAirway批次的上皮电阻值(TEER)使用EVOM2上皮电压计和Endohm电极室(世界精密仪器公司)测量。. A minimum TEER of 300 Ohm • cm² is required for QC release.

Sterility: 在整个生产过程中使用的所有介质都要进行无菌检查. 维持培养基不加抗生素孵育1周，检查无菌性. 用于运输的24孔板上的琼脂糖凝胶也孵育1周，并检查任何污染迹象

Screening for pathogens: 所有细胞都经过筛选，使用PCR检测HIV、乙肝和丙肝呈阴性. 然而，没有任何已知的测试方法可以完全保证细胞是无病原体的. Thus, 这些产品和所有人类衍生产品应按照CDC-NIH手册中建议的BSL-2(生物安全等级2)或更高级别处理, “Biosafety in microbiological and biomedical laboratories,” 1998. 如需进一步帮助，请联系您的现场安全专员或hg8868皇冠国际技术服务

Notification of lot failure: If a tissue lot fails our QC or sterility testing, 我们会通知顾客，并在适当情况下免费更换纸巾. Because our QC and sterility testing is done post-shipment, 在一般情况下，我们会尽快发出通知, TEER failures will be notified by Wednesday 5 p.m.; sterility failures will be notified within 8 days of shipment)