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The primary objective of three-dimensional cell culture is to bridge the gap between the in vitro and in vivo contexts. Furthermore, the application of three-dimensional cell models can help surmount obstacles associated with transferring experimental findings to various species, consequently reducing the need for animal testing.
One such variant of 3D cell culture is spheroid culture, which offers a dependable in vitro model for biomedical research. The inherent three-dimensional arrangement aids in establishing robust cell-cell and cell-matrix interactions, closely mimicking the in vivo microenvironment. Spheroid cultures find extensive utility in drug development, toxicological investigations, as well as cancer and stem cell studies, thus enhancing effectiveness and dependability within these stages of preclinical research.
The BIOFLOAT™ 96-well and 384-well plates from faCellitate surpass current products with their remarkable non-stick attributes. They accelerate spheroid formation, taking just a few hours, thereby reducing experimental duration. The resulting spheroids exhibit exceptional uniformity, ensuring consistent and flawless data. The shape of the wells effectively thwart the development of cell satellites and uneven clusters. The dependable surface quality of BIOFLOAT™ establishes the plates as top-tier choices for cultivating spheroids and organoids, even those cell types that struggle to form spheroids on other available substrates (e.g., primary cells).
BIOFLOAT™ plates find application across diverse domains of biomedical investigation, including but not limited to cancer and stem cell research. They play a crucial role in the preliminary stages of drug development as well as in toxicological inquiries. Their utilization enhance the effectiveness and dependability of preclinical cellular models tailored for these specific purposes.
The surface offered is of exceptional quality, featuring highly-defined characteristics that repel both cells and proteins effectively. Its complete inertness ensures consistent, high-quality performance. The benefits are multiple, including accelerated spheroid generation, consistently reproducible data across various cell lines, and the prevention of irregular cell satellites and aggregates. Notably scratch-resistant and robust, the surface treatment maintains its integrity even under demanding conditions. Additionally, the coating solution is versatile, compatible with a wide array of material substrates without compromising the device’s original geometry.
Processing of Raw Materials
⋅ The product is devoid of any raw materials sourced from animals or biological origins, ensuring the absence of TSE/BSE concerns.
⋅The polymer raw materials utilized for plate coating underwent a comprehensive assessment for quality consistency through GPC, GC-MS, and NMR spectroscopy.
⋅ Employing pipetting robots, the multi-well plates were coated to create sterile, non-pyrogenic/endotoxin-free, non-cytotoxic, DNase-/RNase-/DNA-free surfaces. These coated plates underwent sterilization via electron beam irradiation, adhering to DIN EN ISO 11137 standards.
⋅ The coating brings about a transformation in the plastic surface, rendering it repellent to cells and proteins. This modification facilitates swift formation of spheroids and organoids.
Primary Hepatocyte Spheroid Culture
A novel polymer solution to generate ultra-low cell attachment surfaces and highly uniform spheroids in 3D primary cell cultures
Download the Application Note
Establishment of 3D Spheroids for Cancer Research
A guideline to the establishment of perfect 3D spheroids for cancer research
Download the Application Note